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Criteria for Assessing Applicability to Human Germline Gene Therapy. quenching, the complex formation is observed between the fluorophore. Tumor initiating ability has been accepted as characteristics of cancer stem cells in the field [5]. In the present study, we found that the induced expression of the four transcription factors in melanoma F10-B16 cells significantly increased the number of tumor initiating cells in the cultured cell population. We concluded that the phenotypic remodeling of the cells from melanoma into its stem cells had happened due to induced expression of these factors. This conclusion was further supported by the observation that these four factors also increased the number of tumor initiating cells of melanoma cells in vivo in the present study. It also corroborates data from other published studies showing that these factors remodel the cell phenotype of osteosarcoma and breast cancer cells into osteosarcoma stem cells and breast cancer stem cells respectively [16-18]..